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asparagine to which the oligosaccharide is attached is not peptide-bonded to at least one amino acid residue. Glycopeptidase F F 4450Lot.K702AA. Peptide: N-glycosidase F treatment produced isoforms whose masses varied as a. Because peptide: N-glycosidase F converts asparagine (N) to aspartate (D),. Despite this, enzymic deglycosylation of wild-type protein with peptide N-glycosidase F reveals that glycosylation is not intrinsically required for DNase. span Listed Homes Green class=fFile Format:span PDFAdobe Acrobat - a as HTMLa span class=fFile Format:span PDFAdobe Acrobat - a as

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    Flavobacterium J. Bio. Chem., vol. 265, No.. Samples were then digested with 1.0 unit of N-glycosidase F for 1 h

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    were digested with N-glycosidase F and. run on SDS-PAGE. The N-glycosidase treatment of. the 66-kDa antigen from A-LAK cells yielded a major.

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    protein (50. N-Glycosidase
    F (either 0.09 or 0.18 unit) was then added, and the
    mixture.. The two products may contain N-glycosidase F-resistant sugar residues (i;e.,. The N-Glycosidase Activity of the Protein ME1 Targets. A. Chambery, A. Di Maro, M. M. Monti, F. Stirpe,

    and A. Parente. Western blot analysis